Journal: Nature Communications

Article Title: Chemical reprogramming of fibroblasts into retinal pigment epithelium cells for vision restoration

doi: 10.1038/s41467-025-67104-w

Figure Lengend Snippet: a Representative immunostaining showing that MEF-derived ciRPE cells express ZO-1, Pax6, Rpe65, Mitf, Best1 and Cralbp. Scale bars, 50 μm. b Representative Z-stack confocal micrographs showing ciRPE cells with typical polarized expression of RPE markers. ZO-1 (green) demonstrates apical localization (top), while Best1 (red) shows basolateral localization (bottom). Scale bars, 10 μm. c Representative transmission electron microscopy image of ciRPE cells showing apical microvilli (yellow arrows), melanin granules (red arrows) and tight junctions (black arrows). Scale bars, 1 μm. d Representative confocal micrograph showing phagocytosis of POSs (green) by ciRPEs. The apical sides of ciRPE cells are stained with ZO-1(violet), whereas nuclei are counterstained with DAPI (blue). Scale bars, 50 μm. e Apical and basal secretion of PEDF and VEGF by MEFs, ciRPE, and pRPE cells cultured on Transwells. Each group was compared to the ciRPE group within apical and basal compartments ( n = 6 independent biological samples per group). f Representative morphological images showing dome structures formed by ciRPE cells during in vitro culture. The red arrows indicate the dome morphology observed under different phase-contrast microscopy conditions. Scale bars, 50 μm. g TEER measurements of MEFs, ciRPE, and pRPE cells over time in culture ( n = 6 independent biological samples per group). Data are mean ± SD. One-way ANOVA was used to assess statistical significance. Three independent experiments were performed with similar results and representative results are shown. Source data are provided as a Source Data file.

Article Snippet: Detailed procedures were followed according to the instructions of the PEDF ELISA kit (CUSABIO, CSB-E08820m and CSB-E08818h) and the VEGF ELISA kit (CUSABIO, CSB-E04756m and CSB-E11718h).

Techniques: Immunostaining, Derivative Assay, Expressing, Transmission Assay, Electron Microscopy, Staining, Cell Culture, In Vitro, Microscopy

Journal: Nature Communications

Article Title: Chemical reprogramming of fibroblasts into retinal pigment epithelium cells for vision restoration

doi: 10.1038/s41467-025-67104-w

Figure Lengend Snippet: a Candidate small molecules identified by preliminary screening with scRCF for reprogramming HEFs into OV. b Schematic diagram of the protocol for the reprogramming of HEFs into hciRPE cells, along with representative morphological changes at indicated time points. HM represents the HEF medium, while RM represents the reprogramming medium and DM refers to the differentiation/maturation medium. Scale bar, 300 μm. c FACS purification of reprogrammed BEST1-EGFP + hciRPE cells. d Representative optical microscopy and TEM images of hciRPE cells showing melanin granules (red arrows). Scale bars, 1 μm. e qRT-PCR analysis showing the expression of RPE-associated genes at the indicated time points during reprogramming ( n = 3 independent biological samples per group). f Representative immunostaining analysis showing positive expression of ZO-1, RPE65, MITF and BEST1 in the BEST1-EGFP - HEFs-derived hciRPE cells. Scale bar, 20 μm. g PCA of samples from day 0, day 12, day 24 and day 38 (hciRPE) of celluar reprogramming, and the control primary hRPE cells. h Heatmap showing differentially expressed genes in HEF to hciRPE cell reprogramming samples at indicated time points. The number above heatmap indicates independent biological replicates. Representative genes (left side of the heatmap) and associated GO (right side of the heatmap) for each block are shown. Red and blue indicate upregulated and downregulated genes, respectively. Differential expression was analyzed using the R package limma (v3.58.1) following normalization with edgeR (v4.0.16). Significantly changed genes were defined by |log₂ fold change| > 1.5 and adjusted p < 0.01 (Benjamini-Hochberg correction). i Polarized secretion of VEGF and PEDF from the apical and basal sides of hciRPE cells grown on Transwells ( n = 6 independent biological samples per group). j TEER in hciRPE cells for 30 days. p values indicate comparisons between adjacent time points ( n = 5 independent biological samples per group). Data are mean ± SD. Statistical analyses were performed using one-way ANOVA ( e ) and unpaired, two-tailed Student’s t- test ( i , j ). Three independent experiments were performed with similar results and representative results are shown. Source data are provided as a Source Data file.

Article Snippet: Detailed procedures were followed according to the instructions of the PEDF ELISA kit (CUSABIO, CSB-E08820m and CSB-E08818h) and the VEGF ELISA kit (CUSABIO, CSB-E04756m and CSB-E11718h).

Techniques: Purification, Microscopy, Quantitative RT-PCR, Expressing, Immunostaining, Derivative Assay, Control, Blocking Assay, Quantitative Proteomics, Two Tailed Test

Journal: Stem Cells International

Article Title: Neurotrophic Effect of Adipose Tissue-Derived Stem Cells on Erectile Function Recovery by Pigment Epithelium-Derived Factor Secretion in a Rat Model of Cavernous Nerve Injury

doi: 10.1155/2016/5161248

Figure Lengend Snippet: PEDF in different passages of rat ADSC-conditioned medium. Five samples per passage were analyzed using a rat PEDF ELISA kit. The different passages of the rat ADSCs secreted a detectable level of PEDF in cultured serum-free DMEM/F12 medium. ∗ P < 0.05 compared with the DMEM/F12 medium group only. PEDF = pigment epithelium-derived factor; ADSCs = adipose tissue-derived stem cells; DMEM/F12 = Dulbecco's Modified Eagle's Medium/F12.

Article Snippet: The culture supernatant from the ADSCs was subsequently collected and analyzed using a rat PEDF ELISA kit (CUSABIO, Wuhan, Hubei province, China) according to the manufacturer's instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture, Derivative Assay, Modification

Journal: Stem Cells International

Article Title: Neurotrophic Effect of Adipose Tissue-Derived Stem Cells on Erectile Function Recovery by Pigment Epithelium-Derived Factor Secretion in a Rat Model of Cavernous Nerve Injury

doi: 10.1155/2016/5161248

Figure Lengend Snippet: Western blotting analyses of PEDF, p-Akt, and p-eNOS protein expression in the rat penile tissues of all groups. (a) Representative Western blot and (b) quantitative analysis identified increased PEDF expression in the bilateral cavernous nerve injury (BCNI) group compared with the sham group at day 14, which recovered to the background level at day 28. ∗ P < 0.05. (c)-(d) Increased PEDF expression in the ADSCs treatment group compared with the other groups at day 28. ∗ P < 0.05. (e)-(f) Increased p-Akt expression in the ADSCs group compared with the other groups at day 28 and increased p-Akt expression in the sham group compared with the bilateral cavernous nerve injury and PBS groups. ∗ P < 0.05 and # P < 0.05. (g-h) Increased p-eNOS expression in the ADSCs treatment group compared with the other groups at day 28. ∗ P < 0.05. The data were normalized to GAPDH protein expression. n = 6 per group. p-Akt = phosphorylated Akt; p-eNOS = phosphorylated endothelial nitric oxide synthase; GAPDH = glyceraldehyde 3-phosphate dehydrogenase.

Article Snippet: The culture supernatant from the ADSCs was subsequently collected and analyzed using a rat PEDF ELISA kit (CUSABIO, Wuhan, Hubei province, China) according to the manufacturer's instructions.

Techniques: Western Blot, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Effect of Hypoxia on Pulmonary Endothelial Cells from Bleomycin-Induced Pulmonary Fibrosis Model Mice

doi: 10.3390/ijms23168996

Figure Lengend Snippet: Fibrotic mediator proteins released from endothelial cells. Protein levels of TGF-β1 ( A ), CTGF ( B ), PDGF-B ( C ), PDGF-C ( D ), and PDGF-D ( E ) released from endothelial cells were quantified by ELISA. Data are means ± standard error of the mean for three mice. * p < 0.05.

Article Snippet: Proteins released from endothelial cells were quantified in samples of conditioned medium using ELISA kits for TGF-β1 (R&D Systems, Minneapolis, MN, USA), PDGF-B (R&D Systems), PDGF-C (Cloud-Clone Corp, Wuhan, China), PDGF-D (MyBiosource Inc., San Diego, CA, USA) or CTGF (Cusabio Life Science, Wuhan, China) according to the manufacturers’ protocols.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Oncotarget

Article Title: Malignant ascites: a source of therapeutic protein against ovarian cancer?

doi: 10.18632/oncotarget.27185

Figure Lengend Snippet: ( A ) PEDF mRNA expression in ovarian control or cancer cells. *** p < 0.005 ( B ). PEDF protein level in serum of control, ovarian cancer patients, and in the acellular fraction of ascites obtained from ovarian cancer patients. * p < 0.05.

Article Snippet: PEDF protein was quantified using a human PEDF ELISA kit (BioProductsMD, XpressBio Europe) following the provider’s instructions.

Techniques: Expressing, Control

Journal: Oncotarget

Article Title: Malignant ascites: a source of therapeutic protein against ovarian cancer?

doi: 10.18632/oncotarget.27185

Figure Lengend Snippet: ( A ) Dose-response effect of recombinant PEDF (rPEDF) on SKOV3 cell viability. The 50% inhibitory concentration (IC50) of recombinant PEDF was calculated using Compusyn software.( B ) Determination of the minimal rPEDF concentration allowing additional effect with paclitaxel (Ptx at 100 nM, for 48h) on SKOV3 cell viability.

Article Snippet: PEDF protein was quantified using a human PEDF ELISA kit (BioProductsMD, XpressBio Europe) following the provider’s instructions.

Techniques: Recombinant, Concentration Assay, Software

Journal: Oncotarget

Article Title: Malignant ascites: a source of therapeutic protein against ovarian cancer?

doi: 10.18632/oncotarget.27185

Figure Lengend Snippet: ( A ) Anti-PEDF antibodies (light grey bars) or control IgG (dark grey bars) were added in culture medium of cells treated with 100 nM Ptx. FBS: fetal bovine serum. ( B ) Western blot analysis of PEDF in FBS and ascites ( C ) Western blot analysis of PEDF in ascites, PEDF-depleted ascites (A-PEDF) and purified PEDF (pPEDF). Left panel: Ponceau red staining of membrane; Right panel: PEDF immunoblotting. ( D ) Effect of ascites-PEDF on viability of cells treated with 100 nM Ptx (for 48h) compared to FBS-PEDF. ( E ) Effect of PEDF depletion from ascites on Ptx treated SKOV3-derived tumor growth. ** p < 0.01.

Article Snippet: PEDF protein was quantified using a human PEDF ELISA kit (BioProductsMD, XpressBio Europe) following the provider’s instructions.

Techniques: Control, Western Blot, Purification, Staining, Membrane, Derivative Assay

Journal: Oncotarget

Article Title: Malignant ascites: a source of therapeutic protein against ovarian cancer?

doi: 10.18632/oncotarget.27185

Figure Lengend Snippet: ( A ) Evaluation of Ptx treatment in combination with ascites, PEDF-depleted ascites (ascites-PEDF) or purified PEDF (pPEDF) treatment on COV318-derived tumor growth. ( B ) Evaluation of recombinant PEDF (rPEDF) or purified PEDF (pPEDF) treatment on COV318-derived tumor growth. * p < 0.05 and ** p < 0.01.

Article Snippet: PEDF protein was quantified using a human PEDF ELISA kit (BioProductsMD, XpressBio Europe) following the provider’s instructions.

Techniques: Purification, Derivative Assay, Recombinant

Journal: Oncotarget

Article Title: Malignant ascites: a source of therapeutic protein against ovarian cancer?

doi: 10.18632/oncotarget.27185

Figure Lengend Snippet: ( A ) GFP staining of SKOV3 cells stably transfected with PEDF or control transposon at passage 52 (P52). Magnification: ×200. ( B ) PEDF mRNA level in SKOV3 cells stably transfected with PEDF transposon at different passages (P). ( C ) Time-course of PEDF secretion in culture medium of SKOV3 cells stably transfected with PEDF transposon (grey) or control transposon (black) during 96 h of culture.

Article Snippet: PEDF protein was quantified using a human PEDF ELISA kit (BioProductsMD, XpressBio Europe) following the provider’s instructions.

Techniques: Staining, Stable Transfection, Transfection, Control

Journal: Oncotarget

Article Title: Malignant ascites: a source of therapeutic protein against ovarian cancer?

doi: 10.18632/oncotarget.27185

Figure Lengend Snippet: ( A ) Growth kinetic of PEDF-SKOV3 (grey) or CTRL-SKOV3-derived tumor (black). ( B ) Photographs (upper panel; magnification ×16.5) and Hemalun Eosin staining (middle panel; magnification ×100) of CTRL-SKOV3 and PEDF-SKOV3-derived tumors at EDD13.5. Enlargement of the black squares was presented in the lower panels (magnification ×400). Tumors are indicated by black arrows, and CAM and tumor vessels by white arrows.

Article Snippet: PEDF protein was quantified using a human PEDF ELISA kit (BioProductsMD, XpressBio Europe) following the provider’s instructions.

Techniques: Derivative Assay, Staining

Journal: PLoS ONE

Article Title: Diosmin Alleviates Retinal Edema by Protecting the Blood-Retinal Barrier and Reducing Retinal Vascular Permeability during Ischemia/Reperfusion Injury

doi: 10.1371/journal.pone.0061794

Figure Lengend Snippet: (A) Twenty-four hours after ischemia, there was a significant increase in VEGF expression and a slight increase in PEDF expression in the vehicle group compared with the control group; diosmin treatment reversed this trend. (B) Seven days after ischemia, the VEGF and PEDF protein expression returned to normal in both the vehicle and diosmin groups. (C) The VEGF/PEDF ratio for the three groups 24 h and 7 d after ischemia. Data are expressed as the mean±SD ( n = 8). ** P <0.01, *** P <0.001.

Article Snippet: In each sample, the concentrations of VEGF (Rat VEGF Quantikine ELISA Kit; R&D Systems, Minneapolis, MN) and PEDF (Rat pigment epithelium-derived factor, PEDF ELISA Kit; EIAab ®, Wuhan, China) were measured with ELISA kits according to the respective manufacturer’s protocol.

Techniques: Expressing, Control

Journal: PLoS ONE

Article Title: Diosmin Alleviates Retinal Edema by Protecting the Blood-Retinal Barrier and Reducing Retinal Vascular Permeability during Ischemia/Reperfusion Injury

doi: 10.1371/journal.pone.0061794

Figure Lengend Snippet: No significant immunoreactivity for VEGF and PEDF was found in control retina. I/R injury induced a stronger immunoreactivity for VEGF in retina and diosmin administration relieved this trend. The PEDF immunoreactivity was nearly similar to the control group after 24 h retinal ischemia, while diosmin administration immensely increased this immunoreactivity. Scale bar, 100 µm.

Article Snippet: In each sample, the concentrations of VEGF (Rat VEGF Quantikine ELISA Kit; R&D Systems, Minneapolis, MN) and PEDF (Rat pigment epithelium-derived factor, PEDF ELISA Kit; EIAab ®, Wuhan, China) were measured with ELISA kits according to the respective manufacturer’s protocol.

Techniques: Control